Journal: Scientific Reports

Article Title: Plasma Donors in the Southwestern United States Positively Contribute to the Diverse Therapeutic Antibody Profile of Immune Globulin Products

doi: 10.1038/s41598-020-63794-y

Figure Lengend Snippet: Anti-HAV Ig titers of plasma pools segregated by donor center. Plots demonstrate the spread of observed anti-HAV Ig titers measured by ELISA. The box whiskers denote the minimum and maximum titers; the bottom and top borders of a box represent the first and third quartile markers, respectively; the line within each box denotes the median, and the (+) symbol denotes the mean. (*) Mean is significantly greater than those of San Diego-CA, Midwest City-OK, Provo-UT, and Clarksville-TN, centers (p < 0.001; ANOVA, Tukey’s HSD). (#) Mean is significantly greater than those of Provo-UT, and Clarksville-TN, centers (p < 0.0001; ANOVA, Tukey’s HSD).

Article Snippet: A commercially-available enzyme-linked immunosorbent assay (ELISA) (Bio-Rad MONOLISATM Anti-HAV EIA Kit, cat#72496) was used to estimate the anti-HAV Ig (IgG and IgM) titer in a plasma sample.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Plasma Donors in the Southwestern United States Positively Contribute to the Diverse Therapeutic Antibody Profile of Immune Globulin Products

doi: 10.1038/s41598-020-63794-y

Figure Lengend Snippet: Anti-MeV and anti-CMV IgG titers of plasma pools from select donor centers. Plots demonstrate the spread of anti-MeV and anti-CMV IgG ELISA observations—reported as signal-to-cutoff (S·Co −1 )—in plasma pools from high-titer (El Paso-TX) and mid-titer (Midwest City-OK) donor centers. The box whiskers denote the minimum and maximum titers; the bottom and top borders of a box represent the first and third quartile markers, respectively; the line within each box denotes the median, and the (+) symbol denotes the mean. (*) Mean is greater than that of the El Paso, TX, center (p < 0.0001; unpaired two-tail t test).

Article Snippet: A commercially-available enzyme-linked immunosorbent assay (ELISA) (Bio-Rad MONOLISATM Anti-HAV EIA Kit, cat#72496) was used to estimate the anti-HAV Ig (IgG and IgM) titer in a plasma sample.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Plasma Donors in the Southwestern United States Positively Contribute to the Diverse Therapeutic Antibody Profile of Immune Globulin Products

doi: 10.1038/s41598-020-63794-y

Figure Lengend Snippet: Spread of anti-HAV Ig titers of individual plasma units composing pools obtained from high-titer, mid-titer, and low-titer donor centers. Plots demonstrate the spread of anti-HAV Ig titers measured by ELISA in plasma pools from high-titer (El Paso-TX and McAllen-TX), mid-titer (Midwest City-OK), and low-titer (Clarksville, TN) donor centers. The box whiskers denote the minimum and maximum titers; the bottom and top borders of a box represent the first and third quartile markers, respectively; the line within each box denotes the median, and the (+) symbol denotes the mean.

Article Snippet: A commercially-available enzyme-linked immunosorbent assay (ELISA) (Bio-Rad MONOLISATM Anti-HAV EIA Kit, cat#72496) was used to estimate the anti-HAV Ig (IgG and IgM) titer in a plasma sample.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay

Journal: Molecules

Article Title: Isolation and Characterization of Two Chalcone Derivatives with Anti-Hepatitis B Virus Activity from the Endemic Socotraen Dracaena cinnabari (Dragon’s Blood Tree)

doi: 10.3390/molecules27030952

Figure Lengend Snippet: Dose- and time-dependent inhibitory effect of compound 1 on HBsAg expression in HepG2.2.15 culture. The antigen expression in the culture medium was measured using an ELISA to determine the anti-HBV activity of different concentrations of compound 1 . Lamivudine (2.0 µM) was used as the positive control. Data are presented as the mean ± standard error of the mean ( n = 3), * p < 0.001 vs. positive control group.

Article Snippet: The secreted HBsAg was determined using an enzyme immunoassay (ELISA) kit (Monolisa HBsAg ULTRA, BioRad, Hercules, CA, USA) according to the manufacturer’s instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Activity Assay, Positive Control

Journal: Molecules

Article Title: Isolation and Characterization of Two Chalcone Derivatives with Anti-Hepatitis B Virus Activity from the Endemic Socotraen Dracaena cinnabari (Dragon’s Blood Tree)

doi: 10.3390/molecules27030952

Figure Lengend Snippet: Dose- and time-dependent inhibitory effect of compound 2 on HBsAg expression in HepG2.2.15 culture. The antigen expression in the culture medium was measured using an ELISA to determine the anti-HBV activity of different concentrations of compound 2 . Lamivudine (2.0 µM) was used as the positive control. Data are presented as the mean ± standard error of the mean ( n = 3), * p < 0.001 vs. positive control group.

Article Snippet: The secreted HBsAg was determined using an enzyme immunoassay (ELISA) kit (Monolisa HBsAg ULTRA, BioRad, Hercules, CA, USA) according to the manufacturer’s instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Activity Assay, Positive Control

Journal: Molecules

Article Title: Isolation and Characterization of Two Chalcone Derivatives with Anti-Hepatitis B Virus Activity from the Endemic Socotraen Dracaena cinnabari (Dragon’s Blood Tree)

doi: 10.3390/molecules27030952

Figure Lengend Snippet: Effect of compounds 1 and 2 on time-dependent downregulation of HBeAg expression in HepG2.2.15 culture. The antigen expression in the culture medium was measured using an ELISA. Lamivudine (2.0 μM) was used as the positive control. Data are presented as the mean ± standard error of the mean, * p < 0.001, day 5 vs. day 3.

Article Snippet: The secreted HBsAg was determined using an enzyme immunoassay (ELISA) kit (Monolisa HBsAg ULTRA, BioRad, Hercules, CA, USA) according to the manufacturer’s instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Positive Control

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: List of ELISA tests, cell proliferation, and protein extraction kits used. For ELISA tests, dilution of the supernatants or cell lysate samples used and detection limits are also reported.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Enzyme-linked Immunosorbent Assay, Protein Extraction

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: C-Kit (CD117) mRNA (a, b, c) and protein (d, e, f) expression after ESW treatment in primary bronchial fibroblasts of COPD patients (a, d), primary bronchial fibroblasts of control smokers (b, e), and bronchial epithelial cells (c, f). In bronchial epithelium (16HBE) c-Kit increased at mRNA (c) and protein (f) levels. In primary bronchial fibroblasts of COPD patients, c-Kit increased at protein level (d). T -test was used for comparative purposes, and p values are reported in the graphs.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Expressing

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: Photomicrographs showing thyroid transcription factor-1 (TTF-1) expression (panels a, b), c-Kit (CD117) (c, d), and proliferating cell nuclear antigen (PCNA) (e, f) in the peripheral lung tissue of a representative patient with chronic obstructive pulmonary disease (COPD). Arrows indicate positively stained cells mainly located in the alveolar septa. Bars = 50 microns.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Expressing, Staining

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: Photomicrographs showing alveolar type II epithelial cells (TTF-1+ cells, red color) coexpressing c-Kit (CD117) (brown color) (a, b) and PCNA (brown color) (c, d) in the peripheral lung tissue of a representative patient with COPD. Positive double-stained cells can be recognized in the alveolar septa, even though their presence was only rarely observed. Arrows indicate positively stained cells located in the alveolar septa. Bars = 50 microns.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Staining